Showing items 1 - 3 of 3

Effects of bisphenol a on neonatal cardiomyocytes beating rate and morphology

- Zatilfarihiah Rasdi, Noorul Izzati Hanafi, Siti Hamimah Sheikh Abdul Kadir, Sharaniza Ab. Rahim, Rosfaiizah Siran, Narimah Abdul Hamid Hasani, Syed Baharom Syed Ahmad Fuad

  • Creator: Zatilfarihiah Rasdi , Noorul Izzati Hanafi , Siti Hamimah Sheikh Abdul Kadir , Sharaniza Ab. Rahim , Rosfaiizah Siran , Narimah Abdul Hamid Hasani , Syed Baharom Syed Ahmad Fuad
  • Date: 2018
  • Subjects: Chemical engineering
  • Language: ENG
  • Type: Journal Article
  • Identifier: vital:121580 , ISSN 2180–3722 , valet-20190318-094056
  • Full Text: false
  • Description: Bisphenol A (BPA) has been utilised excessively at a global capacity of 2.9 billion kg/year. It is widely used in manufacturing polycarbonate polymers and epoxy resins. Hence, humans are potentially exposed to this chemical substance in their daily life. As a typical endocrine disruptor, BPA exhibits detectable hormone-like properties. Many studies have been linking BPA exposure in humans with the risk of developing cardiovascular disease, however the direct exposure of BPA on cardiomyocytes beating rates and morphology have not been entirely explored. Therefore, in this study, we aimed to investigate the effects of BPA on cells structure and function of neonatal rat cardiomyocytes culture. Cardiomyocytes were isolated from 0 to 2 days old newborn rats and treated with 0.001 to 100 ìM concentration of BPA. All cardiomyocytes were subjected to immunostaining, beating frequency assessment assay, MTS assay and Scanning Electron microscopy (SEM). In immunostaining, cardiomyocytes showed positive staining for F-actin. This staining allows identification of the cells thus differentiate cardiomyocytes from other cell types. Significance effects of BPA on cardiomyocytes were observed in MTS assay (p<0.05) and beating rates (p<0.01). Significant reduction (48%-64%, ± 1.5280) was observed in beating rate of cardiomyocytes exposed to 0.1 to 100 ìM of BPA. Meanwhile in MTS assay, significant reduction (54%, 0.067 ± 0.0026) in cell viability was observed in cells exposed to 0.1 ìM of BPA only. Interestingly, under SEM, cardiomyocytes showed altered cell surface homogeneity after BPA exposure. Exposure of 0.1 to 100 ìM BPA lead to flatten of cardiomyocytes cell surface and blurring of the cell borders. This study offers an in vitro evidence of BPA effects on cardiomyocytes morphology and beating rates, thus suggest the potential adverse effect of BPA exposure. However, further investigation would be required to understand how BPA effects normal cells morphology and beating rates of heart cells.

Effects of bisphenol a on neonatal cardiomyocytes beating rate and morphology

- Zatilfarihiah Rasdi, Noorul Izzati Hanafi, Siti Hamimah Sheikh Abdul Kadir, Sharaniza Ab. Rahim, Rosfaiizah Siran, Mohd. Hafiz Dzarfan Othman, Roziana Kamaludin, Narimah Abdul Hamid Hasani, Syed Baharom Syed Ahmad Fuad

Ursodeoxycholic acid regulates caspase-9 and ros production in protecting cardiomyocytes against hypoxia

- Noorul Izzati Hanafi, Siti Hamimah Sheikh Abdul Kadir, Anis Syamimi Mohamed, Julina Md. Noor, Nora Julianna Osman, Rosfaiizah Siran, Sharaniza Ab. Rahim, Narimah Abdul Hamid Hasani

  • Creator: Noorul Izzati Hanafi , Siti Hamimah Sheikh Abdul Kadir , Anis Syamimi Mohamed , Julina Md. Noor , Nora Julianna Osman , Rosfaiizah Siran , Sharaniza Ab. Rahim , Narimah Abdul Hamid Hasani
  • Date: 2017
  • Subjects: Biosciences and medical engineering
  • Language: ENG
  • Type: Journal Article
  • Identifier: vital:105811 , ISSN 2180-3722 , valet-20180129-16390
  • Full Text: false
  • Description: Ursodeoxycholic acid (UDCA) is known as a therapeutic agent in treating cholestasis and liver diseases. Recently, UDCA has been suggested as a therapeutic drug for heart related diseases. Cardioprotective effect of UDCA against the development of ischemia has been studied. Yet, the mechanism of UDCA-cardioprotection is not clearly understood. Therefore, this study aimed to elucidate the mechanisms of UDCA cardioprotection against hypoxia by investigating the expression of caspase -3/-9 and ROS generation using an in vitro hypoxic heart model. A newborn (0-2 days old) rat heart was isolated for primary cell culture of cardiomyocytes. Hypoxia was chemically induced by using CoCl2. Cardiomyocytes were then incubated with UDCA. The treated cardiomyocytes were subjected for ROS generation detection assay, QuantiGene Plex assay for caspase-3/-9 gene expression and ELISA for caspase-3/-9 protein expression. The data were analyzed by using sample paired t-test and One-way ANOVA. Our results showed that UDCA abolishes the effects on CoCl2 in ROS production and UDCA downregulates caspase-9 protein expression in CoCl2 treated cardiomyocytes. This study provides an insight of UDCA in protecting cardiomyocytes against hypoxia mediated by anti-apoptosis mechanism.
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